First Cytogenetic Study of Puff-Faced Water Snake, <i>Homalopsis buccata</i> (Squamata, Colubridae) by Conventional Staining, Ag-NOR Banding and GTG-Banding Techniques

Fluorescence and Giemsa Banding Techniques

The C group chromosomes in man cannot be distinguished solely on morphological grounds, thus, the nature of an extra C chromosome remains unknown unless it is an X chromosome, which can be identified by its late DNA-replication pattern. Extra autosomes belonging to the C group have been observed with or without mosaicism in a wide variety of blood disorders (cf, de la Chapelle et al, 1970; Hell...

A study of professional radiation hazards in CT scan and nuclear medicine workers using GTG-banding and solid stain

  Background: CT scan and nuclear medicine exams deliver a great part of medical exposures. This study examined professional radiation hazards in CT scan and nuclear medicine workers.   Methods : In a cross sectional study 30 occupationally exposed workers and 7 controls (all from personnel of a laboratory) were selected. Physical dosimetry was performed for exposed workers. Blood samples were ...

A Rare Case of Klinefelter Syndrome Patient with Quintuple Mosaic Karyotype, Diagnosed by GTG-Banding and FISH

First Molecular Cytogenetic High Resolution Characterization of the NIH 3T3 Cell Line by Murine Multicolor Banding

Since being established in 1963, the murine fibroblast cell line NIH 3T3 has been used in thousands of studies. NIH 3T3 immortalized spontaneously and became tetraploid shortly after its establishment. Here we report the first molecular cytogenetic characterization of NIH 3T3 using fluorescence in situ hybridization based multicolor banding (mcb). Overall, a complex rearranged karyotype present...

Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining

The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chr...